RNA Incorporation (Pyrimidine Analog Misincorporation)

RNA incorporation of pyrimidine analogs refers to the process by which synthetic or modified pyrimidine nucleoside analogs are mistakenly incorporated into RNA molecules during transcription, in place of their natural counterparts (cytidine and uridine). This misincorporation can disrupt normal RNA function and is a key mechanism underlying the cytotoxic effects of several chemotherapeutic agents, most notably 5-fluorouracil (5-FU). This also represents an emerging strategy for antibiotic development due to its high barrier for resistance evolution among pathogens.

Other names

Pyrimidine Analog Misincorporation5-Fluorouracil Incorporation into RNARNA misincorporation

Pyrimidine analog phosphorylated to triphosphate form and incorporated into RNA during transcription, leading to impaired mRNA processing/translation and cell death.