Spindle pole body component 25 homolog (SPC25)

SPC25 is a structural protein that functions as an essential component of the NDC80 kinetochore complex, a heterotetramer consisting of NDC80, NUF2, SPC24, and SPC25 proteins. The NDC80 complex acts at the core of the kinetochore machinery, anchoring the centromere of each chromosome to spindle microtubules—a critical step for accurate chromosome segregation during mitosis. The SPC25 subunit, together with SPC24, forms one end of the complex and binds to centromere-associated adaptor proteins, effectively linking the microtubule-binding activity of the complex to the chromosome. The NDC80 complex exhibits structural flexibility, allowing it to regulate its affinity for microtubule plus-ends and participate in both structural assembly and signaling functions like the spindle assembly checkpoint (SAC). SPC25 is highly conserved and indispensable for proper chromosome alignment, segregation, and error correction in mitosis. Dysregulation or mutation of SPC25 and related kinetochore complex proteins can lead to mitotic defects and chromosome instability, observed in cancers and other proliferative diseases. Currently, SPC25 is considered more of a molecular complex component than a direct drug target or classical biomarker, but its function is central to the cellular division machinery and pathology associated with mitotic errors.

Other names

Kinetochore protein Spc25SPBC25AD024hSpc25MGC222282600017H08RikNDC80 kinetochore complex componentSpindle pole body component 25 homolog

Not applicable; no drugs directly targeting SPC25. Mechanisms would theoretically center on disruption of chromosome segregation or spindle assembly checkpoint by modulating the NDC80 complex as a whole.