Target DNA sequence

"Target DNA sequence" refers to a specific region of DNA that is the subject of manipulation or analysis in molecular biology techniques, such as PCR, sequencing, or genome editing[1][3][7][9]. In PCR applications, it is the DNA region amplified by primer pairs; in CRISPR systems, the target DNA sequence is what is recognized and edited by the CRISPR-Cas effector complex[1]. In sequencing technologies, such as Sanger or next-generation sequencing, the term designates the particular DNA stretch to be determined base-by-base[7][9]. "Target DNA sequence" is not a molecule, protein, or functional gene product, and thus is not classified in the same way as typical therapeutic targets. This term is generic, not unique or specific: it should be replaced by the actual gene, locus, or DNA element of interest according to context.

Other names

target sequenceDNA target regiontarget nucleic acid sequence