Triosephosphate Isomerase (Trypanosoma cruzi)

Triosephosphate isomerase (TIM) from Trypanosoma cruzi (TcTIM) is a crucial enzyme in the glycolytic pathway of this protozoan parasite, which causes Chagas disease. It catalyzes the reversible interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (GAP). TcTIM is considered an essential enzyme for parasite survival and has been validated as a promising drug target for anti-Chagas therapies. Inhibitors targeting TcTIM have shown potent trypanosomicidal activity with minimal toxicity toward mammalian cells due to structural differences. Inhibition leads to disruption of glycolysis and accumulation of toxic metabolites, ultimately impairing parasite viability.

Inhibition of triosephosphate isomerase, disruption of glycolysis, accumulation of methylglyoxal and advanced glycation end products.