tRNA-uridine aminocarboxypropyltransferase 1 (DTWD1)

tRNA-uridine aminocarboxypropyltransferase 1 (DTWD1) is an enzyme that catalyzes the formation of 3-(3-amino-3-carboxypropyl)uridine (acp³U) at position 20 in the D-loop of certain cytoplasmic tRNAs[4][5]. This post-transcriptional tRNA modification is important for tRNA structure and function, particularly related to translation efficiency and growth in mammalian cells[2][3]. DTWD1 is localized in the nucleus[1][4][5], and knockout studies in human cells indicate it is primarily responsible for acp³U20 formation in distinct tRNAs (notably tRNA^Cys and tRNA^Tyr), with overlap and partial redundancy with DTWD2[2][3]. Loss of both DTWD1 and DTWD2 causes severe growth defects in culture, demonstrating the physiological significance of this tRNA modification; however, single knockout of DTWD1 does not strongly impair cell viability[2][3]. No direct evidence currently links DTWD1 as a classical therapeutic target (such as a druggable receptor, transporter, or enzyme directly modulated by pharmacological agents), although it is a critical component in cellular RNA biology.

Other names

DTW domain containing 1DTW domain-containing protein 1MDS009MGC111207x 009 protein