Type II DNA Topoisomerase (None)

Type II DNA topoisomerases are essential enzymes that manage the topology of DNA by introducing transient double-stranded breaks in the DNA helix. This allows them to resolve tangles, supercoils, knots, and catenanes that arise during vital cellular processes such as replication, transcription, recombination, and chromosome segregation. Type II topoisomerases are major targets for anticancer agents. Drugs like etoposide exploit their mechanism by stabilizing the transient double-strand break intermediate—leading to cytotoxicity especially in rapidly dividing cells such as cancer cells.

Stabilization of the transient double-strand break intermediate.