pcDNA-NCF2

pcDNA-NCF2 is a recombinant DNA expression plasmid designed to overexpress the Neutrophil Cytoplasmic Factor 2 (NCF2) protein in mammalian cells. NCF2, also known as p67-phox, is a critical cytosolic subunit of the NADPH oxidase complex, which is responsible for the generation of reactive oxygen species (ROS). In the context of cardiovascular research, pcDNA-NCF2 is utilized as a molecular tool to investigate the pathological role of NCF2 upregulation in endothelial dysfunction. Studies have demonstrated that overexpression of NCF2 via this plasmid inhibits the PI3K/Akt/Nrf2 signaling pathway, thereby promoting ferroptosis and inflammatory responses in human umbilical vein endothelial cells (HUVECs). It is primarily used in preclinical research to model the progression of atherosclerosis and to validate the mechanism of action of potential therapeutic agents that target the NCF2-mediated oxidative stress pathway.

Other names

NCF2 expression plasmidNCF-2 expression plasmidNCF 2 expression plasmidpcDNA3.1-NCF2pcDNA-3.1-NCF2pcDNA 3.1-NCF2